Monday 21st October

Studio 2 Digitalisation

Chair: Krist Gernaey

10.30 Comparison of vibrational spectroscopy methods for real-time monitoring of anaerobic fermentation. M. Mauricio Iglesias

10.45 Real-time cell population analysis in bioreactors using deep learning enabled in situ microscopy. J.-S. Guez

11.00 A “Legacy Zymer’s” Reflections on Industrial Digital Transformation at Novonesis. J. Price

11.15 Real-time state estimation in non-linear adsorption chromatography using Kalman filters. D. Espinoza

11.30 Industrial digital scale-up: Development of a versatile modelling platform M.O. Kaiser Albæk

Studio 4 Biotechnology and metabolic engineering

Chair: John Kavanagh

10.30 Enhancing Xylose Utilization in Bioprocessing: Insights from XUT1 Gene Functional Characterization from Candida tropicalis. S. de Souza Queiroz

10.45 High-precision profiling of population dynamics and metabolites unravels joint biotransformation of natural products in cellulosic co-cultures. A. Palacio-Barrera

11.00 Proteome reduction as a strategy to improve the performance of cell factories. A. R. Lara

11.15 Demonstrating the capabilities of KIWI-Biolab’s robotic ecosystem by orchestration of model-mased DoEs and fast in-depth analytics for the development of recombinant protein processes. P. Neubauer

11.30Utilization of spent sulfite liquor as substrate and inducer for protein production in engineered Corynebacterium glutamicum strains. G. Seibold

Studio 2: Digitalisation

Chair: Ulrich Krühne

13.00 The BioProcessNexus: One step towards free and accessible techno economic bioprocess models. M. Medl

13.15 A Physics -Informed Neural Network (PINNs) Framework for Bioreactor Hybrid Modelling. M. Kumar Thirugnanasambandam

13.30 Uncertainty-aware real-time forecasting of pilot-scale bubble column aeration using ensemble hybrid model. P. Jul-Rasmussen

13.45 The more, the merrier? A quest for an efficient compartmentalization strategy for CFD-based analyses of bioreactors. H. Maldonado De Leon

14.00 Advanced simulation of large-scale bioreactors. J. Le Nepvou De Carfort

14.15 Creating a Digital Twin for a Pilot-Plant Disk Centrifuge in GFPuv Production, A. A. Malanca

Studio 3: Biocatalysis

Chair Bruno Bühler

13.00 Development of Enzymes from Extremophiles for the Degradation of Plastics and Potential Generatio of Useful Metabolites. J. Asenjo

13.15 Continuous synthesis of furfurylamine in a falling film microflow reactor with integrated product separation M. Božinović

13.30 Localisation of lipases inside pickering emulsions – Investigation through adsorption behaviour M. Seiler

13.45 Model-assisted Design and Optimization of a Biocatalytical Cofactor Regeneration Process in a Pressurized Packed-Bed Reactor. L. Arndt

14.00 Process intensification guided by techno-economic analysis for the bioconversion of rutin. Z. Kádár

14.15 Structural and biochemical characterization of vanillin aminotransferases for the biological valorization of lignin. I.Mendes Bonfim

Studio 4: Biotechnology and metabolic engineering

Chair: Peter Neubauer

13.00 Populations under stress: assessment of morphology and physiology heterogeneity in mono- and co-cultivation. S. Junne

13.15 Automated adaptive laboratory evolution of Clostridium kluyveri enables growth at high CO partial pressures. V. Burgmaier

13.30 Metabolic Engineering of new Streptomyces sp. from Extreme Environments for Novel Antibiotics, Anticancer and Antifungal Drugs. B. Andrews

13.45 Enhanced productivity of filamentous pellets investigated with new tools for elucidation of oxygen uptake and morphology. Z. Justyna Kozanecka

14.00 Selection and characterization of microbiomes under the same ecological stress. M. Catalão

14.15Use of bacterial co-culture to overcome the toxicity of lignocellulosic hydrolysates. S. I. Mussatto

Studio 2: FBM Scaling, Gradients and Heterogenity

Chair: John Woodley

10.30-10.40 Welcome by Bjarke Bak Christensen

10.40-11.05 The start of green transition by the use of fermentation of large volume food and material proteins. P. Falholt

11.05-11.30 Gradients in fermentations conditions can impact process performance by leading to decreased yields in large-scale fermentation processes. G. Nadal-Rey

11.30 Optimizing seed train design: Enhancing productivity in biomanufacturing scale-up. L Munkler

Studio 3: Novel upstream and downstream process concepts

Chair: Carina L. Gargola

10.30 Photoautotrophic production of DHA and EPA enriched biomass by co-culturing golden brown and green microalgae. A.-L. Thurn

10.45 Manufacturing of recombinant disulphide-bonded peptides in Escherichia coli using CASPON™ technology. B. Striedner

11.00 Expansion of T-cells Using a Perfused Microfluidic Approach. O. Derevianko

11.15 Upcycling of PET-based plastic waste into biodegradable polyhydroxyalkanoates (PHA) using mixed microbial cultures. P. Concórdio-Reis

11.30 Bioprocess monitoring with miniaturized multi-parameter electrochemical sensors. A. Hasanzadeh

Studio 4: Circular bioeconomy and processing

Chair: Solange L. Mussatto

10.30 All-in-one: Direct conversion of cellulose to erythro-isocitric acid by a non-engineered strain of Talaromyces verruculosus. I. Schlembach

10.45 PEM Electrocatalysis in a Stirred-Tank Bioreactor Enables Growth of Clostridium ragsdalei with CO₂ and Electrons. I. Schwarz

11.00 Bringing food waste back to the food value chain using SustainMAX® technology. P. Madhusudan Bapat

11.15 Integrating process modeling and impacts into an automated sustainability assessment toolbox of bio-based products. S. Sukumara

11.30 Towards Sustainable Food Security: Exploiting Synthetic Microbial Communities for Protein Production from Dairy Industry side streams. B. Delmoitié

Studio 2: FBM Innovation for Biomanufactoring

Chair: Andreas Worberg

13.00-13.20 Planetary Health at the BioInnovation Institute Maria Henriques De Jesus, BII

13.20-13.40 Next-generation food innovation
Johan Agrell, Alfa Laval

13.40-14.00 Food Innovation with Filamentous Fungi
Leonie Johanna Jahn, DTU

14.00-14.20 Valorization of end-of-life waste streams to high-value products. P. Reitzer

14.20-14.40 Breaking the cost barrier – biomanufacturing with FAST continuous extractive fermentation
Eric van der Meer, DAB

Studio 3: Digitalisation

Chair: TBA

13.00 Deep Hybrid Modelling of a Supercritical CO₂ Extraction Process. R. Agharafeie

13.15 Bioprocessing 5.0: From Knowledge Graphs to Cognitive Digital Threads. M. Nicolas Cruz Bournazou

13.30 Applying a Modified DevOps Workflow to Address Challenges in Developing Digitalisation Tools for Chemical and Biochemical Manufacturing. M. Stevnsborg

13.45 A Novel Machine-Learning-Based Scaling Tool for Bioreactors Using CFD Simulation Data. C. Witz

14.00 Merging metabolic networks with deep neural networks under the SBML standard Abstract. J. Pinto

14.15 Process Modeling accelerates bioprocess development and enables digital biomanufacturing. M. Krippl

Studio 4: Biopharmaceutical Process

Chair: Raquel Aires-Barros

13.00 Rational bioprocess development and optimalisation for cultured Red Blood Cells (cRBCs). L. van Der Wielen

13.15 Techno-economic Optimization under Uncertainty for
Pharmaceutical Processes: Ibuprofen. T. Asrav

13.30 Enhancement of mRNA quality for improved vaccine development. A. Rita Santos

13.45 Towards cfDNA extraction from plasma for liquid biopsy applications – partitioning behavior of short DNA. R. Meutelet

14.00 Flocculants for reduction of (high-risk) host cell proteins during primary recovery of monoclonal antibody production processes. A.-C. Frank

14.15 Biomanufacturing Decisions with Data and Models. P. Ramin

Studio 2: Biopharmaceutical process

Chair: Tiffany Rau

10.30 Automatic kLa Determination in Stirred Tank Reactors by Model-Based Design of Experiments. A. H. Valdeira Caetano

10.45 A Hierarchical Approach for Evaluation of Lost Throughput in API Manufacturing. T. Overgaard

11.00 CFD-guided scale-down for end-in-mind bioreactor development: from 2000 L to 2 L. M. Segami

11.15 Vibrio natriegens as a new host for plasmid DNA production. D. Miguel Prazeres

11.30 Purification and characterization of recombinant neuraminidase for non-seasonal vaccine against Influenza virus. F. De Mathia

11.45 Efficient Media and Buffer Formulation Development with Mixture Experiments using Space Filling Designs and Machine Learning. T. Rau

Studio 3: Industrial water and wastewater technology

Chair: TBA

10.30 Immobilization of laccase from Lentinus sajor-caju on chitosan-clay beads and applications on sulfamethoxazole degradation. H. Sá

10.45 Sustainable power generation from salinity gradients via Pressure Retarded Osmosis (PRO): Membrane modification for improved performance. M. Malankowska

11.00 Modelling partial nitration/anammox processes in a full-scale aerobic granular sludge reactor treating reject water. X. Flores-Alsina

11.15 Unveiling the water-energy nexus: Wastewater reuse for district heating applications. F. Sousa Braga

11.30 Bio-release of toxic elements from mining waste. A. Pawlowska

11.45 A preliminary study on the growth of mushroom mycelia via submerged fermentation utilizing a cheap agro-industrial waste. E. Akpari

Studio 4: Bioenergy

Chair: Tony Kiss

10.30 Unveiling biochar’s role in Anaerobic Digestion: myths and facts for enhanced biogas production and sulfate inhibition mitigation. G. Vayena

10.45 Conversion off Oxalic-Acid Impregnated Biomass into Glucose and Bioethanol with the Assistance of Microwave-Irradiation. R. Ceaser

11.00 Computational Simulation of the Pressure-Retarded Osmosis: a Multi-Scale Multi-Physics Membrane-Based Process. M. Mohammadi Amin

11.15 Modeling and Simulation of Biological Water-Gas Shift Reaction in Trickle Bed Reactor and Comparison With CSTR. S. Dutta

11.30 Effect of feeding regime and pH on thermophilic acidogenic fermentations from food waste. L. Vulart

11.45 Maximizing bioenergy production from agro-industrial and municipal wastes in Jalisco: analysis of feedstock effects on the biogas yield of anaerobic co-digestions. M. S. G. Hernández

Studio 2: Biopharmaceutical process

Chair: Arne Staby

13.00 Microbial Process Transfer at Boehringer Ingelheim. E.M. Wlaschitz

13.15 Chemotools: a modern open-source Python software for endto-end PAT applications. P. Cabaneros

13.30 Novel dual-stage cross-flow filtration-based setup for virus-like particle capture. A. Dietrich

13.45 Autonomous operation and quality monitoring of a continuous antibody downstream process. J. K. Lorek

14.00 Hybrid model for mesenchymal stem cell cultivation processes
incorporating seeding heterogeneity. K. Hirono

14.15 Novel filtration technology allows for
simpler and more efficient
separation/recovery of api from biomass and downstream processing. A. Poulsen

Studio 3: Bioprocess for food engineering/precision fermentation

Chair: Marcel Ottens

13.00 Bottlenecks and Opportunities in Cultivated Meat Production. J. Pereira

13.15 Production of Proteins by Precision Fermentation. P. Falholt

13.30 Establishing a High-Throughput Co-Culture Platform to Systematically Screen Diverse Strptomyces Species for new Natural Products. D. Miriam Schütterle

13.45 Modelling of Transport Phenomena and process induced quality Changes During Baking of Soft-Baked Goods. A. H. Feyissa

14.00 The impact of common fermentation medium salts on local holdup in bubble column bioreactors. C. Haringa

14.15 Bubble Column measurements and modelling. J. Kavanagh

Studio 4: Education/New (bio)materials for process engineering

Chair: TBA

13.00 Project Based Teaching of Biochemical Engineering. J. Kavanagh

13.15 Bioprocess Design Education at TU Delft. T. Kiss

13.30 ChatGMP: a Digital Audit Tool for Good Manufacturing Practices. F. Caccavale

13.45 Biosynthesis of Polyhydroxyalkanoates from Lignin Monomers by   Halophiles: High-Throughput Screening and Understanding Limitations. V. Andhalkar

14.00 Fermentative production and purification of L-(+)-lactic acid: assessing the potential of tree and shrub species growing on marginal land. L. Schroedter

14.15 Novel applications in a redesigned 3D-printed micro bubble column reactor. G. Schultz